Tumor growth and angiogenesis is impaired in CIB1 knockout mice

Main Article Content

Mohamed A Zayed Weiping Yuan Dan Chalothorn James E Faber Leslie V Parise

Abstract

Background

Pathological angiogenesis contributes to various ocular, malignant, and inflammatory disorders, emphasizing the need to understand this process more precisely on a molecular level. Previously we found that CIB1, a 22 kDa regulatory protein, plays a critical role in endothelial cell function, angiogenic growth factor-mediated cellular functions, PAK1 activation, MMP-2 expression, and in vivoischemia-induced angiogenesis. Since pathological angiogenesis is highly dependent on many of these same processes, we hypothesized that CIB1 may also regulate tumor-induced angiogenesis.

Methods

To test this hypothesis, we allografted either murine B16 melanoma or Lewis lung carcinoma cells into WT and CIB1-KO mice, and monitored tumor growth, morphology, histology, and intra-tumoral microvessel density.

Results

Allografted melanoma tumors that developed in CIB1-KO mice were smaller in volume, had a distinct necrotic appearance, and had significantly less intra-tumoral microvessel density. Similarly, allografted Lewis lung carcinoma tumors in CIB1-KO mice were smaller in volume and mass, and appeared to have decreased perfusion. Intra-tumoral hemorrhage, necrosis, and perivascular fibrosis were also increased in tumors that developed in CIB1-KO mice.

Conclusions

These findings suggest that, in addition to its other functions, CIB1 plays a critical role in facilitating tumor growth and tumor-induced angiogenesis.

Article Details

How to Cite
ZAYED, Mohamed A et al. Tumor growth and angiogenesis is impaired in CIB1 knockout mice. Vascular Cell, [S.l.], v. 2, n. 1, p. 17, aug. 2010. ISSN 2045-824X. Available at: <https://vascularcell.com/index.php/vc/article/view/10.1186-2040-2384-2-17>. Date accessed: 15 june 2024. doi: http://dx.doi.org/10.1186/2040-2384-2-17.
Section
Original Research