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BackgroundBurkitt's lymphoma (BL) is an aggressive Non-Hodgkin lymphoma. Epstein-Barr Virus (EBV) is able to transform B cells and is a causative infectious agent in BL. The precise role of EBV in lymphoma progression is still unclear. Most investigations have concentrated on cell autonomous functions of EBV in B cells. Functions of the local environment in BL progression have rarely been studied, mainly due to the lack of appropriate in vivo models. Therefore, we inoculated different human BL cell-lines onto the chorioallantoic membrane (CAM) of embryonic day 10 (ED10) chick embryos and re-incubated until ED14 and ED17.
All cell-lines formed solid tumors. However, we observed strong differences in the behavior of EBV +and EBV -cell-lines. Tumor borders of EBV +cells were very fuzzy and numerous cells migrated into the CAM. In EBV -tumors, the borders were much better defined. In contrast to EBV -cells, the EBV +cells induced massive immigration of chick leukocytes at the tumor borders and the development of granulation tissue with large numbers of blood vessels and lymphatics, although the expression of pro- and anti-angiogenic forms of Vascular Endothelial Growth Factors/receptors was the same in all BL cell-lines tested. The EBV +cell-lines massively disseminated via the lymphatics and completely occluded them.
Our data suggest that the EBV +cells attract pro-angiogenic leukocytes, which then induce secondary tumor-stroma interactions contributing to the progression of BL. We show that the CAM is a highly suitable in vivo model to study the differential behavior of BL cell-lines.
How to Cite
BECKER, Jürgen et al. Specific tumor-stroma interactions of EBV-positive Burkitt's lymphoma cells in the chick chorioallantoic membrane. Vascular Cell, [S.l.], v. 4, n. 1, p. 3, mar. 2012. ISSN 2045-824X. Available at: <https://vascularcell.com/index.php/vc/article/view/10.1186-2045-824X-4-3>. Date accessed: 19 oct. 2019. doi: http://dx.doi.org/10.1186/2045-824X-4-3.